Studies on the Rumen Flagellate Sphaeromonas communis

Studies on the rumen flagellate Neocallimastix frontalis.

The vast increase in the population density of the rumen flagellate Neocallimastix frontalis shortly after the host animal has commenced eating is caused by stimulation of a reproductive body on a vegetative phase of the organism to differentiate and liberate the flagellates. The stimulant is a component of the host's diet. The vegetative stage of N. frontalis bears a strong morphological resem...

The rumen flagellate Callimastix frontalis: does sequestration occur?

Degradation of maize stem by two rumen fungal species, Piromyces communis and Caecomyces communis, in pure cultures or in association with cellulolytic bacteria.

Two species of rumen fungi, Piromyces (Piromonas) communis FL and Caecomyces (Sphaeromonas) communis FG10, were cultured alone or in association with the cellulolytic bacteria Ruminococcus flavefaciens or Fibrobacter succinogenes on maize stem. A kinetic study of the degradation of the substrate was then made. After 48 h of culture, all non-lignified tissues observed by scanning electron micros...

Studies on Bovine Rumen Bacterial Urease 1

The propert ies of rumen urease were studied. In the absence of high concentrations of dithiothreitol enzyme activity was rapidly lost; in its presence, the enzyme could be solubilized and purified in high yield. Maximum activity was observed between pH 7 and 8.5. The Km for urea with partially purified enzyme was .004 M with Vmax of 300 to 400 #moles/mg/hr. No divalent metal ion requirement or...

Chemical modification studies on Ricinus communis (Castor Bean) agglutinin.

Ricinus communis agglutinin was subjected to various chemical treatments and the effect on its hemagglutinating and saccharide-binding properties was studied. Acetylation, succinylation and citraconylation led to a complete loss in the activity of the agglutinin, whereas reductive methylation had no effect on the activity, showing that charged amino groups were involved in the hemagglutinating ...